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Convenient, economical method testing for avocado sunblotch viroid

Nucleic acid hybridization assay (ASBVd) is found in avocado growing regions worldwide. Trees infected with ASBVd can result in a loss of yield and / or production of unmarketable fruit. However, symptoms are not always present making pro-active testing a critical disease control measure.

Agdia’s ASBVd Nucleic Acid Hybridization Assay is a convenient and economical method for testing hundreds of trees at once. Its high sensitivity allows combining up to 8 leaves from each of 4 trees, or a total of 32 leaves, for analysis. The assay’s limit of detection is approximately 12.5 picograms of in vitro expressed ASBVd RNA.

End-users are only required to prepare the sample extracts and spot them onto a specially treated membrane, which is provided in the kit. Once this is completed, the membrane is then shipped to Agdia’s Testing Services Department where the chemiluminescent hybridization is performed by highly trained technicians. This analysis is included the price. Results are usually reported within two to four business days of our laboratory receiving the membrane for analysis.

Also Available for Avocado
For customers that wish to mail samples to Agdia Testing Services, we now offer an avocado screen that includes testing for:
  • Avocado sunblotch viroid (ASBVd)
  • Phytophthora (Phyt)
  • Xanthomonas campestris (Xc)
For more information on how to send in samples, please contact us [email protected].

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